An image processing pipeline for electron cryo‐tomography in <scp>RELION</scp> ‐5

Electron tomography of frozen, hydrated samples allows structure determination of macromolecular complexes that are embedded in complex environments. Provided that the target complexes may be localised in noisy, three-dimensional tomographic reconstructions, averaging images of multiple instances of these molecules can lead to structures with sufficient resolution for de novo atomic modelling. Although many research groups have contributed image processing tools for these tasks, a lack of standardisation and interoperability represents a barrier for newcomers to the field. Here, we present an image processing pipeline for electron tomography data in RELION-5, with functionality ranging from the import of unprocessed movies to the automated building of atomic models in the final maps. Our explicit definition of metadata items that describe the steps of our pipeline has been designed for interoperability with other software tools and provides a framework for further standardisation.

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Publication Details

Journal
FEBS Open Bio
Published
2024-08-15
DOI
https://doi.org/10.1002/2211-5463.13873
Citations
163
Primary Topic
Advanced Electron Microscopy Techniques and Applications
Type
article
Field-Weighted Citation Impact
85.05

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article

An image processing pipeline for electron cryo‐tomography in RELION ‐5

Bogdan Toader, Jasenko Zivanov, Tanmay A. M. Bharat, Dari Kimanius et al.
163 citations
FEBS Open Bio
Advanced Electron Microscopy Techniques and Applications
85.05
article

An image processing pipeline for electron cryo‐tomography in RELION ‐5

Bogdan Toader, Jasenko Zivanov, Tanmay A. M. Bharat, Dari Kimanius, Euan Pyle, Alister Burt, Andriko von Kügelgen, Sjors H. W. Scheres, Rangana Warshamanage
article en
163 citations

Abstract

Electron tomography of frozen, hydrated samples allows structure determination of macromolecular complexes that are embedded in complex environments. Provided that the target complexes may be localised in noisy, three-dimensional tomographic reconstructions, averaging images of multiple instances of these molecules can lead to structures with sufficient resolution for de novo atomic modelling. Although many research groups have contributed image processing tools for these tasks, a lack of standardisation and interoperability represents a barrier for newcomers to the field. Here, we present an image processing pipeline for electron tomography data in RELION-5, with functionality ranging from the import of unprocessed movies to the automated building of atomic models in the final maps. Our explicit definition of metadata items that describe the steps of our pipeline has been designed for interoperability with other software tools and provides a framework for further standardisation.

FEBS Open BioVol. 14(11)
Science and Technology Facilities Council (GB), University of Pittsburgh (US), MRC Laboratory of Molecular Biology (GB), The Francis Crick Institute (GB), Institute of Structural and Molecular Biology (GB), Birkbeck, University of London (GB)
Pittsburgh Foundation, Leverhulme Trust, Medical Research Council, Biotechnology and Biological Sciences Research Council, H2020 European Research Council
Industry, innovation and infrastructure
Openalex Percentile: Top 0%
Advanced Electron Microscopy Techniques and Applications
85.05
DOIOpen Access PDFOpenAlex
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