EFFECT OF AG-NANOPARTICLE IN BIOCHEMICAL AND PHYSIOLOGICAL CHANGES DURING PROGRESS OF SEED DEVELOPMENT AND MATURATION IN GREEN GRAM

Seed development and maturation comprises of a series of events involving cell division/ histodifferentiation, retains deposition and desiccation.Development of green gram seed has been divided into eight consecutive stages; 5, 10, 15, 20, 25, 30, 35, 40 days after anthesis (DAA).During seed development, chlorophyll was declined, while carbohydrate and protein contents were increased, suggesting their supportive role in germination and early seedling growth.As seeds developed upto 30 days after anthesis did not germinate at all, germination potential and seedling parameters including vigour index were considered for the last two stages of development (35 and 40 DAA) only.The highest germination potential was recorded as 81.24 % and 80.99 %, for seeds developed at the stage of 40 DAA in first and second year respectively.Significantly highest vigour was observed at 40 DAA, when average was made over genotypes and treatments.Seeds of Samrat produced after seed treatment with Ag-NP were of highest vigour status when harvested at 40 DAA.So, for seed purpose ideal harvesting time for greengram is 40 DAA as at that time seed vigour maximum.