Novel Candidate Genes Involved in an Initial Stage of White Striping Development in Broiler Chickens
White striping (WS) is a myopathy characterized by the appearance of white stripes parallel to the muscle fibers in the breast of broiler chickens, composed of adipose and connective tissues. This condition causes economic losses and, although common, its etiology remains poorly understood. Hence, the objective was to identify genes and biological mechanisms involved in the early stages of WS using a paternal broiler line that grows slightly slower than commercial ones, at 35 days of age, through the RNA sequencing of the pectoralis major muscle. Thirty genes were differentially expressed between normal and WS-affected chickens, with 23 upregulated and 7 downregulated in the affected broilers. Of these, 14 genes are novel candidates for WS and are implicated in biological processes related to muscle development (<i>CEPBD</i>, <i>DUSP8</i>, <i>METTL21EP</i>, <i>NELL2</i>, and <i>UBE3D</i>), lipid metabolism (<i>PDK4</i>, <i>DDIT4</i>, <i>FKBP5</i>, <i>DGAT2</i>, <i>LIPG</i>, <i>TDH</i>, and <i>RGCC</i>), and collagen (<i>COL4A5</i> and <i>COL4A6</i>). Genes related to changes in muscle fiber type and the processes of apoptosis, autophagy, proliferation, and differentiation are possibly involved with the initial stage of WS development. In contrast, the genes linked to lipid metabolism and collagen may have their expression altered due to the progression of the myopathy.
Authors
- Mônica Corrêa Ledur (ORCID: https://orcid.org/0000-0002-2645-4735)
- Laila Talarico Dias (ORCID: https://orcid.org/0000-0002-8888-8993)
- M. A. Z. Morés (ORCID: https://orcid.org/0000-0001-9198-8824)
- A. M. G. Ibelli (ORCID: https://orcid.org/0000-0003-0626-8879)
- Gabriel Costa Monteiro Moreira (ORCID: https://orcid.org/0000-0003-3139-1027)
- F. de C. Tavernari (ORCID: https://orcid.org/0000-0002-3161-019X)
- Maurício Egídio Cantão (ORCID: https://orcid.org/0000-0002-5988-6542)
- Lana Teixeira Fernandes (ORCID: https://orcid.org/0000-0001-7610-5843)
- Ana Paula Bastos (ORCID: https://orcid.org/0000-0002-0873-1639)
- Rodrigo de Almeida Teixeira (ORCID: https://orcid.org/0000-0002-5213-6722)
- Suelen Fernandes Padilha (ORCID: https://orcid.org/0000-0002-6123-4105)
- Jane Oliveira Peixoto
Institutions
- Concordia University Wisconsin (US)
- Universidade do Estado de Santa Catarina (BR)
- Université de Versailles Saint-Quentin-en-Yvelines (FR)
- Université Paris-Saclay (FR)
- Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR)
- Universidade Estadual do Centro-Oeste (BR)
- Biologie de la Reproduction, Environnement, Epigénétique et Développement (FR)
- Universidade Federal do Paraná (BR)
Publication Details
- Journal
- Animals
- Published
- 2024-08-16
- DOI
- https://doi.org/10.3390/ani14162379
- Citations
- 2
- Primary Topic
- Animal Genetics and Reproduction
- Type
- article
- Field-Weighted Citation Impact
- 1.10
Funders
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
- Empresa Brasileira de Pesquisa Agropecuária
- Conselho Nacional de Desenvolvimento Científico e Tecnológico
- Fundação de Amparo à Pesquisa e Inovação do Estado de Santa Catarina
- Fundação para a Ciência e a Tecnologia