Fast optical diffraction tomography microscopy with large field of view and lossless pupil beam combination

Optical diffractive tomography (ODT) microscopy is a normal wide-field, non-invasive and label-free three-dimensional imaging technology for cells and tissues. The traditional ODT microscopy has a little field-of-view (FOV) of about 80um, which needs to be reconstructed by regions and then spliced for large complex biological samples with sub-millimeter scale. However, there is interference of ringing effect during splicing, which limits its application in sub-millimeter biological samples. In this paper, an ODT microscopy with a wider FOV is proposed. The FOV is 196um, more than three times that of conventional technology, and the photon flux is higher. The results show that the wider-field ODT microscopy has better imaging performance, higher signal-to-noise ratio on the sub-millimeter samples without splicing.