Bio recovering of waste water and agricultural soil polluted by waste industrial oil or waste motor oil

Water and soil are natural resources that can be contaminated by waste industrial oil (WIO) or waste motor oil (WMO).This causes loss of soil fertility and makes it difficult to reuse water for various purposes.The aim of this work was to analyze the bio-remediation of water and soil impacted by 20,000 ppm of WIO or WMO.For this purpose, wastewater and agricultural soil contaminated by WIO or WMO were biostimulated by Tween 20, followed by biostimulation with 2 mineral solutions with different concentrations of monobasic and dibasic phosphates, H 2 O 2 with and without agitation.An experimental design with two controls and four treatments was used.The response variables used to measure the removal of WIO and WMO were the production of CO 2 and the disappearance of aliphatic hydrocarbons by Soxhlet.The experimental results were analyzed by ANOVA-Tukey.The results showed that in both agricultural soil and waste water, the first biostimulation by Tween 20 was necessary to emulsify the aliphatic hydrocarbons of WIO and WMO, followed by biostimulation with mineral solution 1 with the highest amount of phosphate salts, which induced a rapid mineralization of WIO and WMO, both in waste water and soil, which was followed by biostimulation with H 2 O 2 with and without agitation to increase the removal amount of hydrocarbons from WIO and WMO, due to the high hydrocarbon oxidation activity by native aerobic heterotrophic microorganisms in soil and waste water.The results of biostimulated soil and waste water were statistically different compared to nonbiostimulated waste water and agricultural soil where WIO or WMO did not disappear.It was indicated that in both waste water and agricultural soil, the aliphatic hydrocarbons of WIO and WMO were the ones that were removed faster with mechanical agitation and H 2 O 2 .It was detected that in both soil and water, the concentration of WIO and WMO was reduced from 20,000 ppm to 1000 ppm.It is concluded that biostimulation is a useful strategy for the recovery of water waste and agricultural soil impacted by WIO and WMO to allowed to reuse with not risk for humans or animals.