Suppression of NLRP3 inflammasome orchestrates the protective efficacy of tiron against isoprenaline-induced myocardial injury
The major contribution of myocardial damage to global mortalities raises debate regarding the exploration of new therapeutic strategies for its treatment. Therefore, our study investigated the counteracting effect of tiron against isoprenaline (ISO)-mediated cardiac infarction in mice. Tiron was administered to mice for 7 days prior to two consecutive injections of ISO on days 8 and 9 of the treatment protocol. Tiron significantly reduced the levels of CK-MB, LDH, and AST in serum samples of ISO-challenged mice. A considerable increase in the cardiac antioxidant response was observed in tiron-treated mice, as indicated by depletion of MDA and enhancement of antioxidant activities. Furthermore, tiron induced a marked decrease in NLRP3, ASC, and caspase-1 levels accompanied by weak immune reactions of IL-1β, NF-κB, TLR4, and iNOS in the infarct cardiac tissues. Histopathological screening validated these variations observed in the cardiac specimens. Thus, tiron clearly mitigated the oxidative and inflammatory stress by repressing the NLRP3 inflammasome and the TLR4/NF-κB/iNOS signaling cascade.
Authors
- Mohamed El‐Sherbiny (ORCID: https://orcid.org/0000-0002-0814-1743)
- Eman S. El‐Ashry (ORCID: https://orcid.org/0000-0001-7129-7815)
- Rasha Hamed Al‐Serwi (ORCID: https://orcid.org/0000-0002-2121-9269)
- Dalia H. El‐Kashef (ORCID: https://orcid.org/0000-0002-0021-5533)
- Iman Ibrahim (ORCID: https://orcid.org/0000-0002-7408-6113)
- Doaa Abdelrahaman
- Ola A. Habotta (ORCID: https://orcid.org/0000-0003-0067-2838)
- Ateya Megahed Ibrahim (ORCID: https://orcid.org/0000-0001-8192-0017)
- Safwa M. Sorour (ORCID: https://orcid.org/0000-0002-5545-7740)
- Hala Magdy Anwer (ORCID: https://orcid.org/0000-0002-1225-1814)
- Ehab S. Taher
- Rada Olga
- Ostan Mihaela
- Khairiah M. Alwutayed
- Reham M. Ibrahim
- Ahmed Abdeen
Institutions
- Princess Nourah bint Abdulrahman University (SA)
- Alfaisal University (SA)
- Prince Sattam Bin Abdulaziz University (SA)
- Zarqa University (JO)
- Mansoura University (EG)
- Benha University (EG)
- Almaarefa University
- Port Said University (EG)
Publication Details
- Journal
- Frontiers in Pharmacology
- Published
- 2024-08-15
- DOI
- https://doi.org/10.3389/fphar.2024.1379908
- Citations
- 7
- Primary Topic
- Inflammasome and immune disorders
- Type
- article
- Field-Weighted Citation Impact
- 1.64
Funders
- Prince Sattam bin Abdulaziz University
- AlMaarefa University
- Princess Nourah Bint Abdulrahman University
- Zarqa University
- College of Veterinary Medicine and Biomedical Sciences, Texas A and M University