Investigations into the Protein Stabilization of Musts and Wines by Aspergillopepsin under Different Enzymatic and Thermal Treatments

Protein removal with aspergillopepsin in combination with short-time heating has recently emerged as an opportunity for winemakers to prevent protein haze in wine. In this study, we first treated a protein-rich must and wine with two different aspergillopepsin preparations along an identical control treatment without enzyme addition on a pilot plant scale. While treating wines was rather ineffective, treating musts was effective in reducing protein concentrations by up to 86%, leading to haze-stable wines according to the common heat test. Varying thermal loads in further experiments, a rather mild heat treatment of 65 °C for 30 s compared to those in earlier studies was found sufficient for enzymatic protein degradation. Treating must from a fungus-resistant variety (cv. Fidelio) was as successful as treating must from a blend of conventional varieties (cv. Gewürztraminer/Müller-Thurgau). Triangle sensory tests found no clear evidence of an effect on the quality by heat treatments with and without enzyme dosage.

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Journal
ACS Food Science & Technology
Published
2024-08-15
DOI
https://doi.org/10.1021/acsfoodscitech.4c00308
Primary Topic
Fermentation and Sensory Analysis
Type
article
Field-Weighted Citation Impact
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article

Investigations into the Protein Stabilization of Musts and Wines by Aspergillopepsin under Different Enzymatic and Thermal Treatments

Katharina Happel, Wendell Albuquerque, Bernhard Spengler, Parviz Ghezellou et al.
ACS Food Science & Technology
Fermentation and Sensory Analysis
article

Investigations into the Protein Stabilization of Musts and Wines by Aspergillopepsin under Different Enzymatic and Thermal Treatments

Katharina Happel, Wendell Albuquerque, Bernhard Spengler, Parviz Ghezellou, Leif Seidel, Maximilian Freund, Martin Gand, Ralf M. Schweiggert, Holger Zorn, Frank Will, Katrin Runkel
article en

Abstract

Protein removal with aspergillopepsin in combination with short-time heating has recently emerged as an opportunity for winemakers to prevent protein haze in wine. In this study, we first treated a protein-rich must and wine with two different aspergillopepsin preparations along an identical control treatment without enzyme addition on a pilot plant scale. While treating wines was rather ineffective, treating musts was effective in reducing protein concentrations by up to 86%, leading to haze-stable wines according to the common heat test. Varying thermal loads in further experiments, a rather mild heat treatment of 65 °C for 30 s compared to those in earlier studies was found sufficient for enzymatic protein degradation. Treating must from a fungus-resistant variety (cv. Fidelio) was as successful as treating must from a blend of conventional varieties (cv. Gewürztraminer/Müller-Thurgau). Triangle sensory tests found no clear evidence of an effect on the quality by heat treatments with and without enzyme dosage.

ACS Food Science & TechnologyVol. 4(9)
Justus-Liebig-Universität Gießen (DE), Fraunhofer Institute for Molecular Biology and Applied Ecology (DE), Hochschule Geisenheim University (DE)
Deutsche Forschungsgemeinschaft
Openalex Percentile: Top 85%
Fermentation and Sensory Analysis
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