Targeting ALDOA to modulate tumorigenesis and energy metabolism in retinoblastoma

This study aims to elucidate the pivotal role of aldolase A (ALDOA) in retinoblastoma (RB) and evaluate the potential of the ALDOA inhibitor itaconate in impeding RB progression. Utilizing single-cell RNA sequencing, ALDOA consistently exhibits overexpression across diverse cell types, particularly in cone precursor cells, retinoma-like cells, and retinoblastoma-like cells. This heightened expression is validated in RB tissues and cell lines. ALDOA knockdown significantly diminishes RB cell viability, impedes colony formation, and induces notable metabolic alterations. RNA-seq analysis identifies SUSD2, ARHGAP27, and CLK2 as downstream genes associated with ALDOA. The application of itaconate demonstrates efficacy in inhibiting RB cell proliferation, validated through <i>in vitro</i> and <i>in vivo</i> models. This study emphasizes ALDOA as a promising target for innovative RB therapies, with potential implications for altering tumor energy metabolism.

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Publication Details

Journal
iScience
Published
2024-08-15
DOI
https://doi.org/10.1016/j.isci.2024.110725
Citations
6
Primary Topic
Cancer, Hypoxia, and Metabolism
Type
article
Field-Weighted Citation Impact
1.71

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article

Targeting ALDOA to modulate tumorigenesis and energy metabolism in retinoblastoma

Yaoming Liu, Yujun Ma, Hongwei Zhang, Siming Ai et al.
6 citations
iScience
Cancer, Hypoxia, and Metabolism
1.71
article

Targeting ALDOA to modulate tumorigenesis and energy metabolism in retinoblastoma

Yaoming Liu, Yujun Ma, Hongwei Zhang, Siming Ai, Chao Cheng, Jinmiao Li, Shuxia Chen, Cheng Li, Ping Zhang, Xinyue Wang, Zhihui Zhang, Yinghao Wang, Junjie Tang, Rong Lu, Shicai Su, Yuxiang Mao, Yang Gao
article en
6 citations

Abstract

This study aims to elucidate the pivotal role of aldolase A (ALDOA) in retinoblastoma (RB) and evaluate the potential of the ALDOA inhibitor itaconate in impeding RB progression. Utilizing single-cell RNA sequencing, ALDOA consistently exhibits overexpression across diverse cell types, particularly in cone precursor cells, retinoma-like cells, and retinoblastoma-like cells. This heightened expression is validated in RB tissues and cell lines. ALDOA knockdown significantly diminishes RB cell viability, impedes colony formation, and induces notable metabolic alterations. RNA-seq analysis identifies SUSD2, ARHGAP27, and CLK2 as downstream genes associated with ALDOA. The application of itaconate demonstrates efficacy in inhibiting RB cell proliferation, validated through <i>in vitro</i> and <i>in vivo</i> models. This study emphasizes ALDOA as a promising target for innovative RB therapies, with potential implications for altering tumor energy metabolism.

iScienceVol. 27(9)
Sun Yat-sen University (CN)
National Natural Science Foundation of China, Natural Science Foundation of Guangdong Province
Affordable and clean energy
Openalex Percentile: Top 15%
Cancer, Hypoxia, and Metabolism
1.71
DOIOpen Access PDFOpenAlex
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