Protocol for the derivation of primary cancer stem cell lines from human ependymal tumors
Cancer stem cells (CSCs) established from surgical biopsies closely mimic the human context and can be used to investigate disease mechanisms, genetic fitness, and therapeutic evaluation. Here, we present a protocol for the derivation of primary patient-derived CSC lines from ependymal tumors. We describe the necessary steps, from surgical intervention and biopsy to the dissociation of ependymomas to derive cultures. We then detail procedures for cell line propagation and define the characteristics of these primary cancer cell lines. For complete details on the use and execution of this protocol, please refer to Michealraj et al.<sup>1</sup>.
Authors
- Kulandaimanuvel Antony Michealraj (ORCID: https://orcid.org/0000-0002-3967-0816)
- Peter B. Dirks (ORCID: https://orcid.org/0000-0001-5718-6465)
- Cory M. Richman
- Michael D. Taylor (ORCID: https://orcid.org/0000-0001-7009-3466)
Institutions
- University of Pittsburgh (US)
- Baylor College of Medicine (US)
- University of Toronto (CA)
- Hospital for Sick Children (CA)
- Texas Children's Hospital (US)
- Neurological Surgery (US)
Publication Details
- Journal
- STAR Protocols
- Published
- 2024-08-15
- DOI
- https://doi.org/10.1016/j.xpro.2024.103260
- Citations
- 1
- Primary Topic
- Cancer Cells and Metastasis
- Type
- article
- Field-Weighted Citation Impact
- 0.28
Funders
- American Association for Cancer Research
- Genome Canada
- Government of Ontario
- Government of Canada
- Brain Tumour Research
- Stand Up To Cancer
- Canadian Institutes of Health Research