FORMULATION OF BINAHONG LEAVES (ANREDERA CORDIFOLIA) EXTRACT NANOPARTICLE AND EVALUATION OF IN VIVO ANTIDIABETIC ACTIVITY

Objective: Binahong leaves (Anredera cordifolia (Ten.) Steenis) is an herbal plant that is most commonly used to cure various types of diseases in Indonesia, including diabetes. The objective of this study was to conduct a comparative analysis of the antidiabetic activities between binahong leaves extract and binahong leaves extract nanoparticles. Methods: Nanoparticle formulation was carried out using chitosan and sodium alginate polymers as crosslinkers. DDY strain mice with 70% ethanol extract of binahong leaves as test material Binahong leaf extract was tested on alloxan-induced diabetic mice at a dose of 200 mg/kgBW intraperitoneally. The dose of binahong extract used was 200 mg/kgBB and 200 mg/kgBB of chitosan-sodium alginate nanoparticles orally for 14 ds. Results: Obtained extract nanoparticle met the specification, with average sizes of 192.7 nm, zeta potential of+25.3 mV. Extract nanoparticle with a dose equivalent to 200 mg/kgBB binahong leaf extract can reduce blood glucose levels in mice induced by alloxan was better than binahong leaf extract at a dose 200 mg/kgBW with respective percent reduction in blood glucose levels are 45.89% and 33.38%. Conclusion: The formulated extract nanoparticle can enhance the antidiabetic activity of binahong leaves extract.