ENHANCED SULFATED EXOPOLYSACCHARIDE PRODUCTION IN PORPHYRIDIUM PURPUREUM FROM INDONESIA: IMPACT OF CULTURE MEDIUM AND DURATION

Objective: This study investigates the influence of culture medium (Walne and F/2) and culture duration (14 ds and 28 ds) on sulfated Exopolysaccharide (EPS) production in Porphyridium purpureum from Indonesia. EPS sulfate has significant biotechnological potential due to its versatile applications, including in nanotechnological applications and optimizing cultivation conditions is essential to maximize yields. Methods: Cultures of P. purpureum were subjected to two distinct culture media, Walne and F/2, and maintained for two different durations, 14 ds and 28 ds. The microalgal biomass was harvested, and sulfated EPS was extracted using established methods. Statistical analysis was employed to assess the significance of differences between the cultural conditions. The novelty of this study lies in the comprehensive examination of the interactive effects of culture medium composition and duration on sulfated EPS production in P. purpureum. The findings contribute to our understanding of the dynamic responses of P. purpureum to different culture environments. Results: The results of this research reveal a significant increase in sulfated EPS production in the 28 d culture compared to the 14 d culture. Conclusion: This outcome underscores the importance of prolonged culture durations for achieving higher yields of sulfated EPS in P. purpureum.