Cultivation of Halophilic Archaea Halobacterium salinarum
A light-sensitive protein from extremophile archaea Halobacterium salinarum, bacteriorhodopsin (HsBR), has found numerous applications in pharmacology, biotechnology, bioelectronics and other fields [1, 2] due to its ability to convert light energy into a gradient of hydrogen ions across the cell membrane. Despite a wide range of its practical applications, the quantum mechanism of proton transfer remains not fully discovered yet. For further investigation and cost-effective implementation of developments, a higher yield of BR-rich biomass is necessitated. Hereafter we present our findings regarding efficient synthesis of HsBR using its natural host, H. salinarum.
Authors
- Sergei V. Kalenov (ORCID: https://orcid.org/0000-0001-9181-6950)
- Yury L. Ryzhykau (ORCID: https://orcid.org/0000-0003-3719-1464)
- D. D. Kuklina (ORCID: https://orcid.org/0000-0002-9614-5137)
- I.S. Okhrimenko (ORCID: https://orcid.org/0000-0002-1053-2778)
- Anatolii Mikhailov
- E. A. Dronova (ORCID: https://orcid.org/0009-0001-1231-3755)
- A. Yu. Shishkin (ORCID: https://orcid.org/0009-0008-4528-8868)
- I. O. Bezruchko (ORCID: https://orcid.org/0009-0009-5468-6531)
Institutions
- D. Mendeleyev University of Chemical Technology of Russia (RU)
- Moscow Institute of Physics and Technology (RU)
- Joint Institute for Nuclear Research (RU)
- Institute of Cytology (RU)
Publication Details
- Journal
- Physics of Particles and Nuclei Letters
- Published
- 2024-08-01
- DOI
- https://doi.org/10.1134/s1547477124701449
- Citations
- 2
- Primary Topic
- Photoreceptor and optogenetics research
- Type
- article
- Field-Weighted Citation Impact
- 0.54