Study of the Effect of UV Laser Pulse Duration and Wavelength on Fibroblasts

We report the cytotoxic effect of pulse duration and wavelength of UVB laser radiation from Li(Lu,Y)F4:Ce laser on human skin fibroblasts. When performing the MTT test 24 h after irradiation, an increase in the viability parameter was observed. Flow cytometry showed that 80.8 ± 6.9% of cells have a damaged membrane and only 6.2 ± 2.2% reach the stage of late apoptosis, due to which they have the potential to restore the cytoplasmic membrane and subsequently divide.

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Publication Details

Journal
Physics of Particles and Nuclei Letters
Published
2024-08-01
DOI
https://doi.org/10.1134/s1547477124700717
Citations
1
Primary Topic
Laser Applications in Dentistry and Medicine
Type
article
Field-Weighted Citation Impact
0.40
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article

Study of the Effect of UV Laser Pulse Duration and Wavelength on Fibroblasts

D. A. Makarova, Andrey A. Buglak, Т. А. Телегина, N. I. Shamsutdinov et al.
1 citations
Physics of Particles and Nuclei Letters
Laser Applications in Dentistry and Medicine
0.40
article

Study of the Effect of UV Laser Pulse Duration and Wavelength on Fibroblasts

D. A. Makarova, Andrey A. Buglak, Т. А. Телегина, N. I. Shamsutdinov, Pavel Zelenikhin, А. С. Низамутдинов, Y. M. Hamdan
article en
1 citations

Abstract

We report the cytotoxic effect of pulse duration and wavelength of UVB laser radiation from Li(Lu,Y)F4:Ce laser on human skin fibroblasts. When performing the MTT test 24 h after irradiation, an increase in the viability parameter was observed. Flow cytometry showed that 80.8 ± 6.9% of cells have a damaged membrane and only 6.2 ± 2.2% reach the stage of late apoptosis, due to which they have the potential to restore the cytoplasmic membrane and subsequently divide.

Physics of Particles and Nuclei LettersVol. 21(4)
St Petersburg University (RU), Kazan Federal University (RU), A N Bach Institute of Biochemistry (RU)
Openalex Percentile: Top 36%
Laser Applications in Dentistry and Medicine
0.40
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