Biochemical characterization of an exo-polygalacturonase of strain Aspergillus terreus PA3A5T and its application in fruit juice clarification
This study aimed to characterize an exo-PG from Aspergillus terreus PA3A5T and evaluate its application in the clarification of fruit juices. Exo-PG was obtained by submerged fermentation and it was purified by ion-exchange chromatography and gel filtration. The enzyme presented molecular mass of 95.2 kDa, with maximum activity at 70 °C in a pH of 4.0 and remaining stable in an acid pH at 60°C. FeCl2, SnCl2, CoCl2, ZnSO4, NaCl, Al2(SO4)3, MgCl2, MnCl2, and CuSO4 ions activated the enzymatic activity, and PbCl2 inhibited it completely. The value of Km was 1.83 mg mL-1 and Vmax was 335 U mg-1. The A. terreus PA3A5T exo-PG proved to be very effective in the clarification process of the juices, significantly reducing viscosity and color.
Authors
- José Luis da Conceição Silva (ORCID: https://orcid.org/0000-0002-5460-8797)
- Géssica Caroline da Silva (ORCID: https://orcid.org/0000-0002-6704-7415)
- Marina Kimiko Kadowaki (ORCID: https://orcid.org/0000-0002-9174-5811)
- Vitória Maciel Delai (ORCID: https://orcid.org/0000-0003-2682-1248)
- Alexandre Maller (ORCID: https://orcid.org/0000-0002-8065-9184)
- Rita de Cássia Garcia Simão (ORCID: https://orcid.org/0000-0003-2999-1218)
Institutions
- Universidade Estadual do Oeste do Paraná (BR)
Publication Details
- Journal
- Scientia Plena
- Published
- 2024-08-12
- DOI
- https://doi.org/10.14808/sci.plena.2024.071101
- Citations
- 1
- Primary Topic
- Enzyme Production and Characterization
- Type
- article
- Field-Weighted Citation Impact
- 0.39
Funders
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
- Conselho Nacional de Desenvolvimento Científico e Tecnológico
- Fundação Araucária
- Universidade Estadual do Oeste do Paraná