The fluorescence of MM1, leptosperin in Australian and New Zealand Manuka Honeys

The Manuka Marker 1 fluorescence of Australian and New Zealand Manuka honeys at ex 270- em 365 nm correlates to their leptosperin content. Non-Manuka honey varieties did not fluoresce at these wavelengths. A strong correlation R 2 = 0.96 was found between the fluorescence response and leptosperin content by HPLC of Manuka honeys. The highest concentrations of leptosperin were observed in Australian Manuka honey with a maximum value of 2031 mg/kg in comparison to New Zealand Manuka honey 729 mg/kg. In this study, no correlation was observed between leptosperin and methylglyoxal content.