Protocol for isolating extracellular vesicles from pancreatic cancer cells for liver metastatic niche research
Extracellular vesicles (EVs) are secreted, cell-derived, membrane-bound compartments implicated in various diseases for their ability to influence distant targets and as carriers of biomarkers. Here, we present a protocol for separating EVs from mammalian pancreatic cancer cells and their characterization using western blot and electron microscopy. We then demonstrate how they are utilized to affect tumor development in a murine model of metastatic pancreatic cancer including a method to quantify hepatic tumor burden in histologic samples. For complete details on the use and execution of this protocol, please refer to Dudgeon et al.<sup>1</sup>.
Authors
- Crissy Dudgeon (ORCID: https://orcid.org/0000-0001-9010-2569)
- Mélanie Bellina (ORCID: https://orcid.org/0000-0002-7484-6848)
- Darren R. Carpizo (ORCID: https://orcid.org/0000-0002-3824-0160)
- Wade C. Narrow
- Muhammad Sadeqi Nezhad (ORCID: https://orcid.org/0000-0001-6244-385X)
- Orjola Prela
- Anthony Casabianca (ORCID: https://orcid.org/0000-0003-0416-2288)
- Chris Harris (ORCID: https://orcid.org/0000-0003-0340-6674)
- Stephany Fiore
- Patrick Mehlen (ORCID: https://orcid.org/0000-0003-1743-5417)
- Igor Astsaturov (ORCID: https://orcid.org/0000-0002-8613-1890)
- Lan Wang
- Agnès Bernet (ORCID: https://orcid.org/0000-0002-8260-9711)
Institutions
- Université Claude Bernard Lyon 1 (FR)
- Rutgers, The State University of New Jersey (US)
- Fox Chase Cancer Center (US)
- Centre National de la Recherche Scientifique (FR)
- Inserm (FR)
- University of Rochester Medical Center (US)
- Netris Pharma (France) (FR)
- Centre de Recherche en Cancérologie de Lyon (FR)
- Rutgers Cancer Institute (US)
- University of Rochester (US)
Publication Details
- Journal
- STAR Protocols
- Published
- 2024-08-13
- DOI
- https://doi.org/10.1016/j.xpro.2024.103256
- Primary Topic
- Extracellular vesicles in disease
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Pancreatic Cancer Action Network
- Agence Nationale de la Recherche
- Ligue Contre le Cancer
- Institut National Du Cancer