A high-performance digital polymerase chain reaction chip integrated with nanorods-decorated microchannel plate
Abstract In this paper, we report on a digital PCR chip integrated with microchannel plate (MCP) for the quantification of DNA molecules. MCP, a highly porous glass membrane, is employed here as microreactors. The density of the microreactors reaches up to 1600 mm −2 with a total number of 40,000 chambers each in 100 pL volumes embedded in a 5 × 5 mm 2 MCP, which is 100 times larger than that of the conventional microfabricated chips. In addition, the MCP is functionalized with ZnO nanorods to enhance the fluorescence signal. The dynamic range is noted as 10 5 and the detection limit of λDNA is determined to be 1.4 copies/μL.
Authors
- Xi Yang (ORCID: https://orcid.org/0000-0002-8953-8565)
- Zhen Cao (ORCID: https://orcid.org/0000-0002-5440-3628)
- Guangyang Li (ORCID: https://orcid.org/0009-0003-5887-9126)
- Lingyue Xue
- Mingzhu Xu (ORCID: https://orcid.org/0000-0002-6502-9854)
- Xuefeng Xu (ORCID: https://orcid.org/0009-0008-3048-1892)
- A.C. Xiang
- Sixing Hu
Institutions
- Zhejiang University (CN)
Publication Details
- Journal
- Journal of Physics Conference Series
- Published
- 2024-08-01
- DOI
- https://doi.org/10.1088/1742-6596/2809/1/012029
- Primary Topic
- Microfluidic and Capillary Electrophoresis Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.00