Propagating and banking genetically diverse human sapovirus strains using a human duodenal cell line: investigating antigenic differences between strains
The control of human sapovirus, which causes acute gastroenteritis in individuals of all ages, is challenging because of its association with outbreaks similar to those caused by human norovirus. The establishment of conditions for efficient viral propagation of various viral strains is essential for understanding the infection mechanism and identifying potential control methods. In this study, two critical factors for human sapovirus propagation in a conventional human duodenal cell line were identified, and 15 strains of different genotypes that differed at the genetic and antigenic levels were isolated and used to prepare virus stocks. The preparation of virus stocks has not been successful for noroviruses, which belong to the same family as sapoviruses. Securing virus stocks of multiple human sapovirus strains represents a significant advance toward establishing a reliable experimental system that does not depend on limited virus-positive fecal material.
Authors
- T Kobayashi (ORCID: https://orcid.org/0009-0007-7558-4003)
- Michiyo Kataoka (ORCID: https://orcid.org/0000-0002-2654-8803)
- Yuriko Suzaki
- Yasushi Ami (ORCID: https://orcid.org/0000-0001-5507-7265)
- Tetsuo Mita
- Yen Hai Doan (ORCID: https://orcid.org/0000-0002-6782-3541)
- Yuko Okemoto‐Nakamura (ORCID: https://orcid.org/0009-0003-8173-7345)
- Eisuke Tokuoka
- Tetsuya Yoshida (ORCID: https://orcid.org/0000-0002-4520-3640)
- Oka T (ORCID: https://orcid.org/0000-0001-5224-2499)
- Tian‐Cheng Li (ORCID: https://orcid.org/0000-0002-6047-8482)
- Hiroyuki Saito (ORCID: https://orcid.org/0000-0003-2120-059X)
- Hirotaka Takagi (ORCID: https://orcid.org/0000-0002-3489-3105)
- Shinichiro Shibata
- Kenzo Yonemitsu
Institutions
- Nagano University (JP)
- Kumamoto Health Science University (JP)
- National Institute of Infectious Diseases (JP)
- Institute of Public Health (AL)
- Fukuoka Institute of Health and Environmental Sciences (JP)
- Shimane Prefectural Police (JP)
- Nagoya Industrial Science Research Institute (JP)
- Nara Prefectural Institute of Public Health Research Center (JP)
Publication Details
- Journal
- Journal of Virology
- Published
- 2024-08-12
- DOI
- https://doi.org/10.1128/jvi.00639-24
- Citations
- 4
- Primary Topic
- Viral gastroenteritis research and epidemiology
- Type
- article
- Field-Weighted Citation Impact
- 1.56
Funders
- Japan Agency for Medical Research and Development
- Research Promotion Foundation
- Ministry of Health, Labour and Welfare
- Shionogi
- Japan Society for the Promotion of Science