Biomimetic Proteoglycans Strengthen the Pericellular Matrix of Normal and Osteoarthritic Human Cartilage
In osteoarthritis (OA), degradation of cartilage pericellular matrix (PCM), the proteoglycan-rich immediate cell microniche, is a leading event of disease initiation. This study demonstrated that biomimetic proteoglycans (BPGs) can diffuse into human cartilage from both normal and osteoarthritic donors and are preferentially localized within the PCM. Applying immunofluorescence (IF)-guided AFM nanomechanical mapping, we show that this localization of BPGs increases the PCM micromodulus of both normal and OA specimens. These results illustrate the capability of BPGs to integrate with degenerative tissues and support the translational potential of BPGs for treating human OA and other diseases associated with proteoglycan degradation.
Authors
- Anita Li (ORCID: https://orcid.org/0000-0003-3874-3865)
- Elizabeth R. Kahle (ORCID: https://orcid.org/0000-0001-8394-459X)
- Hooman Fallahi (ORCID: https://orcid.org/0000-0002-6676-2919)
- Mary K. Mulcahey (ORCID: https://orcid.org/0000-0001-6125-8169)
- Michele Marcolongo (ORCID: https://orcid.org/0000-0002-1316-5774)
- Annika R. Bergstrom (ORCID: https://orcid.org/0000-0002-1775-092X)
- Lin Han (ORCID: https://orcid.org/0000-0003-4180-1288)
- X. Lucas Lu (ORCID: https://orcid.org/0000-0001-6905-6887)
- Colette E. Trouillot
Institutions
- Loyola University Medical Center (US)
- Drexel University (US)
- Villanova University (US)
- University of Delaware (US)
Publication Details
- Journal
- ACS Biomaterials Science & Engineering
- Published
- 2024-08-12
- DOI
- https://doi.org/10.1021/acsbiomaterials.4c00813
- Citations
- 6
- Primary Topic
- Osteoarthritis Treatment and Mechanisms
- Type
- article
- Field-Weighted Citation Impact
- 2.82
Funders
- National Institute of Arthritis and Musculoskeletal and Skin Diseases
- Division of Civil, Mechanical and Manufacturing Innovation