Distinctive duodenal microbiomes and bile acid profiles in duodenal tumor patients revealed by prospective observational study
The incidence of duodenal tumors (DTs) is increasing. However, the mechanisms underlying its development remain unclear. Environmental factors, including the microbiome and bile acids (BAs), are believed to influence tumor development. Therefore, we conducted a single-center, prospective, observational study to investigate the potential differences between patients with DTs and healthy controls (HCs) based on these factors. In addition, the BAs in the duodenal fluid were measured using liquid chromatography-tandem mass spectrometry. We recruited 41 patients and performed 16S rRNA-seq. There was no difference in the observed ASVs or PCoA plot of Bray-Curtis dissimilarity between the DTs and HCs. The lithocholic acid concentration was significantly lower in the DT group than in the control group. The ratio of CDCA to LCA was significantly higher in patients with DTs. No significant differences in microbiota were observed between DTs and HCs. In patients with DTs, the lithocholic acid concentration in duodenal was significantly lower than in HCs.
Authors
- Kohei Morioka
- Teppei Akimoto (ORCID: https://orcid.org/0000-0002-7472-5210)
- Atsuto Kayashima (ORCID: https://orcid.org/0000-0002-3981-2984)
- Motohiko Kato (ORCID: https://orcid.org/0000-0002-7579-1316)
- Kurato Miyazaki (ORCID: https://orcid.org/0009-0004-1833-7645)
- Teppei Masunaga (ORCID: https://orcid.org/0000-0002-3968-0969)
- Takanori Kanai∥ (ORCID: https://orcid.org/0000-0002-1466-4532)
- Tomohisa Sujino (ORCID: https://orcid.org/0000-0003-0699-6577)
- Atsushi Nakayama (ORCID: https://orcid.org/0000-0001-6487-504X)
- Nobuhiro Nakamoto (ORCID: https://orcid.org/0000-0001-5203-7444)
- Yusaku Takatori (ORCID: https://orcid.org/0000-0003-2403-278X)
- Yoko Kubosawa (ORCID: https://orcid.org/0000-0002-6512-6849)
- Kaoru Takabayashi (ORCID: https://orcid.org/0000-0002-4891-8246)
- Naohisa Yahagi (ORCID: https://orcid.org/0000-0002-9597-4750)
- Mari Mizutani (ORCID: https://orcid.org/0000-0001-5718-6764)
- Kentaro Miyamoto (ORCID: https://orcid.org/0000-0001-5302-2607)
- Noriko Matsuura (ORCID: https://orcid.org/0000-0002-9322-9642)
- Daisuke Minezaki (ORCID: https://orcid.org/0000-0002-0429-4814)
- Akira Honda (ORCID: https://orcid.org/0000-0003-0902-8272)
- Kentaro Iwata
Institutions
- Keio University (JP)
- Keio University Hospital (JP)
- Taiho Pharmaceutical (Japan) (JP)
- Tokyo Medical University Ibaraki Medical Center (JP)
Publication Details
- Journal
- Scientific Reports
- Published
- 2024-08-12
- DOI
- https://doi.org/10.1038/s41598-024-69820-7
- Citations
- 6
- Primary Topic
- Gut microbiota and health
- Type
- article
- Field-Weighted Citation Impact
- 1.40
Funders
- Japan Agency for Medical Research and Development
- Japan Society for the Promotion of Science
- Japan Science and Technology Agency