IDDF2024-ABS-0335 Study of alistipes_finegoldii in predicting clinical remission of UC and preliminary validation

<h3>Background</h3> <i>Alistipes</i> is a class of gram-negative bacteria, which belongs to <i>Bacteroidetes</i>. Studies have indicated its protective effects against liver fibrosis, hypertension, and atrial fibrillation, while it’s implicated in the pathogenesis of colorectal cancer and psychological symptoms of depression. However, there is limited research examining its role in ulcerative colitis (UC). <h3>Methods</h3> Comparing fecal samples from 30 UC patients with 6 healthy donors and 15 UC patients in active with remission to investigate the clinical impact of <i>Alistipes</i> on colitis by using 16S-rDNA high-throughput sequencing. 30 male BALB/c mice were randomly divided into three groups: healthy control (Control), DSS-induced colitis (DSS) and <i>Alistipes_finegoldii</i> intervention (DSS+AF). Colitis was induced by administering 2.5% DSS in drinking water. Control and DSS groups received PBS, while the DSS+AF group received <i>Alistipes_finegoldii</i> (10^8-9 CFU/mL) by gavage for seven consecutive days. The anti-inflammatory effect was evaluated by using disease activity index (DAI), colon shortening, histological damage, and expression of pNF-κB/NF-κB. <h3>Results</h3> In the UC and HD groups, the Firmicutes/Bacteroidetes ratio, a marker of gut dysbiosis, was 2.67 and 3.14. LEfSe analysis revealed a significant enrichment of <i>Alistipes</i> in the HD group, with an LDA Score of 4.05 (<i>P</i>&lt;0.0001). Compared to UC patients, <i>Alistipes</i> was enriched in the gut microbiota of healthy individuals (IDDF2024-ABS-0335 Figure 1 (A). LEfSe analysis between UC and HD). The ratios of <i>Firmicutes</i>/<i>Bacteroidetes</i> were 1.52 and 2.33 in the UC-Ac and UC-Re groups. In LEfSe analysis, the LDA Score of <i>Alistipes</i> in the UC-Re group was 3.52 (<i>P</i>=0.0005). <i>Alistipes</i> was enriched in the gut microbiota of UC patients in remission compared to active (IDDF2024-ABS-0335 Figure 1 (B). LEfSe analysis between UC-Ac and UC-Re). Following intervention with <i>Alistipes_finegoldii</i>, colitic mice exhibited significant improvements in DAI (<i>P</i>&lt;0.0001, <i>P</i>=0.0313), colon shortening (<i>P</i>&lt;0.0001, <i>P</i>=0.0162), histopathological score (<i>P</i>&lt;0.0001, <i>P</i>=0.0009) and activation of NF-κB (<i>P</i>=0.0139, <i>P</i>=0.0136) (IDDF2024-ABS-0335 Figure 1 (C-F). <i>Alistipes_finegoldii</i> ameliorated experimental colitis). <h3>Conclusions</h3> <i>Alistipes</i> may be a potential indicator of clinical remission in UC. <i>Alistipes_finegoldii</i> has shown effectiveness in improving experimental colitis.