IDDF2024-ABS-0019 Dynamic changes in gut microbiota and fecal short-chain fatty acids following colonoscopy: enhancing microbiome stabilization through clostridium butyricum intervention

<h3>Background</h3> Colonoscopy is a classic diagnostic method with possible complications including abdominal pain and diarrhoea. In this study, gut microbiota dynamics and related metabolic products during and after colonoscopy were explored to accelerate gut microbiome balance through probiotics. <h3>Methods</h3> The gut microbiota and fecal short-chain fatty acids (SCFAs) were analyzed in four healthy subjects before and after colonoscopy, along with seven individuals supplemented with <i>Clostridium butyricum</i>. We employed 16S rRNA sequencing and GC-MS to investigate these changes. We also conducted a bioinformatic analysis to explore the<i> buk</i> gene, encoding butyrate kinase, across <i>C. butyricum</i> strains from the human gut. <h3>Results</h3> The gut microbiota and fecal short-chain fatty acids (SCFAs) of four healthy subjects were recovered on the 7th day after colonoscopy. We predicted <i>that Clostridium</i> and other genera might have efficient butyric acid production through bioinformatic analysis of the. Supplementation of seven healthy subjects with <i>Clostridium butyricum</i> after <i>c</i>olonoscopy<i> resulted</i> in a quicker recovery and stabilization of gut microbiota and fecal SCFAs on the third day. <h3>Conclusions</h3> We suggest that supplementation of <i>Clostridium butyricum a</i>fter <i>c</i>olonoscopy should be considered in future routine clinical practice.