Gilvimarinus gilvus sp. nov. and Gilvimarinus algae sp. nov., isolated from kelp seedlings

Two novel rod-shaped, strictly aerobic, non-motile and Gram-stain-negative bacterial strains, designated SDUM040013^T and SDUM040014^T, were isolated from kelp seedlings in Weihai, PR China. Cells of strain SDUM040013^T were 0.3-0.4 µm wide and 0.8-1.8 µm long, catalase-positive and oxidase-positive. Growth of SDUM040013^T was observed at 0-37 °C (optimum, 28-30 °C) and pH 5.5-9 (optimum, pH 8.0) and in the presence of 1-8 % (w/v) NaCl (optimum, 2 %). The DNA G+C content of strain SDUM040013^T was 50.5 %. Strain SDUM040013^T showed the highest 16S rRNA gene sequence similarity (97.1 %) to <i>Gilvimarinus chinensis</i>. Cells of strain SDUM040014^T were 0.4-0.5 µm wide and 1.0-1.4 µm long, catalase-positive and oxidase-positive. Growth of SDUM040014^T was observed at 4-40 °C (optimum, 28-30 °C) and pH 5.5-9 (optimum, pH 8.5) and in the presence of 0-8 % (w/v) NaCl (optimum, 2 %). The DNA G+C content of strain SDUM040014^T was 56.5 %. Strain SDUM040014^T showed the highest 16S rRNA gene sequence similarity (96.2%) to <i>Gilvimarinus polysaccharolyticus</i>. The isoprenoid quinone of both strains was Q-8 and the predominant fatty acids were summed feature 3 (C_{16 : 1} <i> ω</i>7<i>c</i> and/or C_{16 : 1} <i> ω</i>6<i>c</i>), summed feature 8 (C_{18 : 1} <i> ω</i>7<i>c</i>) and C_{16 : 0}. Diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine were the major polar lipids. Given these phenotypic and chemotaxonomic properties, as well as phylogenetic data, strains SDUM040013^T and SDUM040014^T were considered to represent two novel species of the genus <i>Gilvimarinus</i>, for which the names <i>Gilvimarinus gilvus</i> sp. nov. and <i>Gilvimarinus algae</i> sp. nov. are proposed. The type strains are SDUM040013^T (=KCTC 8123^T=MCCC 1H01413^T) and SDUM040014^T (=KCTC 8124^T=MCCC 1H01414^T), respectively.