Multi-view occlusion removal in digital lensless holographic microscopy

A multi-view occlusion removal method for digital lensless holographic microscopy (DLHM) is presented. Multiple DLHM holograms, whose individual reconstructions show occluded or partially occluded sample details, are recorded for different sample placements at its plane. A coordinated addition of the multiple DLHM recordings produces a composite hologram whose reconstruction allows the removal of the occlusions for a given imaging plane while increasing the reconstructed field of view. A theoretical model supports the method and its feasibility is tested with phase bio- and non-bio samples.

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Publication Details

Journal
Optical Engineering
Published
2024-08-12
DOI
https://doi.org/10.1117/1.oe.63.11.111806
Primary Topic
Digital Holography and Microscopy
Type
article
Field-Weighted Citation Impact
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article

Multi-view occlusion removal in digital lensless holographic microscopy

Carlos Buitrago-Duque, Jorge Garcı́a-Sucerquia, Samuel I. Zapata-Valencia
Optical Engineering
Digital Holography and Microscopy
article

Multi-view occlusion removal in digital lensless holographic microscopy

Carlos Buitrago-Duque, Jorge Garcı́a-Sucerquia, Samuel I. Zapata-Valencia
article en

Abstract

A multi-view occlusion removal method for digital lensless holographic microscopy (DLHM) is presented. Multiple DLHM holograms, whose individual reconstructions show occluded or partially occluded sample details, are recorded for different sample placements at its plane. A coordinated addition of the multiple DLHM recordings produces a composite hologram whose reconstruction allows the removal of the occlusions for a given imaging plane while increasing the reconstructed field of view. A theoretical model supports the method and its feasibility is tested with phase bio- and non-bio samples.

Optical EngineeringVol. 63(11)
Universidad Nacional de Colombia (CO)
Openalex Percentile: Top 90%
Digital Holography and Microscopy
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