Microbiological culture versus 16S/18S rRNA gene PCR-sanger sequencing for infectious keratitis: a three-arm, diagnostic cross-sectional study
This study highlights a similar diagnostic performance of direct culture, indirect culture and 16S rRNA PCR for bacterial keratitis, with substantial inter-test concordance. PCR serves as a useful diagnostic adjuvant to culture, particularly in culture-negative cases or those with lesser disease severity (where culture-PCR concordance is lower).
Authors
- Darren Shu Jeng Ting (ORCID: https://orcid.org/0000-0003-1081-1141)
- Harminder Singh Dua (ORCID: https://orcid.org/0000-0002-4683-6917)
- Jennifer L. Cottell
- Jennifer M. Holden
- Michelle M. Lister (ORCID: https://orcid.org/0000-0002-5221-4102)
- Zun Zheng Ong (ORCID: https://orcid.org/0000-0002-3091-1871)
- Imran Mohammed (ORCID: https://orcid.org/0000-0002-8412-0768)
- Yasmeen Hammoudeh
- Lakshmi Suresh (ORCID: https://orcid.org/0009-0009-6204-7043)
- Dalia Galal Said (ORCID: https://orcid.org/0009-0005-2533-7245)
- D. John I. Thomas
Institutions
- University of Nottingham (GB)
- New Cross Hospital (GB)
- Birmingham and Midland Eye Centre (GB)
- Coventry (United Kingdom) (GB)
- Queen's Medical Centre (GB)
- Cardiff University (GB)
- University of Birmingham (GB)
- University of Warwick Science Park (GB)
Publication Details
- Journal
- Frontiers in Medicine
- Published
- 2024-08-12
- DOI
- https://doi.org/10.3389/fmed.2024.1393832
- Citations
- 6
- Primary Topic
- Ocular Infections and Treatments
- Type
- article
- Field-Weighted Citation Impact
- 3.23
Funders
- Medical Research Council