Evaluation of Ocular In-Situ Hydro-gel Formulated prednisolone for Controlling Experimentally Induced Uveitis In Rabbits

One of the major limitations in ophthalmic delivery is the poor bioavailability of drugs from conventional eye drops. pre-corneal drug loss factors include rapid tear turnover, transient residence time, and the relative impermeability of corneal epithelium. occasionally, systemic absorption of drugs may result in systemic side effects. to overcome these issues, controlled drug delivery systems were developed, including different types of in situ gel-forming systems based on different mechanisms (temperature, ph, or ion activated) using different polymers that have been explored for sustained ocular delivery. this study aims to formulate ocular drugs as loaded ionic and ph-activated in situ hydrogel formulas for controlled drug delivery systems aiming to increase the pre-corneal residence time and reduce the frequency of application, limit side effects, and improve patient adherence to therapy. this study will conduct biochemical and ophthalmological tests for combined lecithin, alginic acid, and chitosan-loaded prednisolone eye drops in an animal model of induced uveitis. This study will be conducted to improve ocular bioavailability, and conjunctival sac residence time, reduce frequency of application, and maximize the clinical efficacy of the used topical drug for the treatment of ocular uveitis. A modified formulation of lecithin and combined hydrogel loaded with prednisolone will be formed and evaluated. Animal models of ocular uveitis will be established to test for ophthalmological efficacy of the new formulations. Ocular tissue samples will be obtained for biochemical studies to confirm the clinical efficacy and investigate biochemical changes.