Effect of Biochar and Mycorrhiza Inoculation on Maize Growth, Photosynthesis Activity, and Water Use Efficiency Under Deficient Irrigation Conditions

Climate change-caused water deficiency is a major threat to crop production. Different strategies such as biochar and mycorrhiza are suggested as a solution to improve water storage in the soil and increase plant tolerance to moisture deficiency. This study tested the hypothesis that under deficit irrigation conditions, the combined application of biochar and mycorrhiza can increase maize photosynthesis activity, growth, and water use efficiency (WUE). A greenhouse experiment was established to understand the effect of biochar and mycorrhizal inoculation with deficit irrigation on maize (Zea mays L.) growth, photosynthesis activity, and WUE. A factorial randomized pot experiment was designed using three biochars (B) doses from three different feedstock (DF) and three mycorrhizae (M) species (without mycorrhizae, Rhizophagus clarus and indigenous mycorrhiza), with three different irrigation (I) levels, namely, I100, I75, and I50 respectively 100%, 75%, and 50% of field capacity. For each biochar application, of 0%, 1%, and 2% rates of soil weight were applied. Maize plant dry matter (DM), photosynthesis activities, and leaf water use efficiency (WUEL) were measured. The results showed that deficit irrigation levels, such as I75 and I50 can be used for maize plant growth. Mycorrhizal inoculation increased maize plant growth between 8.2% and 8.9%, whereas biochar increased growth. In addition, mycorrhizal inoculation increased the photosynthesis activity by 9.3%. All the biochars increased maize growth and photosynthesis activity compared to the control, however, poultry manure biochar had the highest growth, photosynthesis activity, and WUE. In addition, statistically, there were no significant differences between B0*M0*I100 and B*M*I50 applications in terms of photosynthesis activity, WUE, and DM. Combined application of biochar and mycorrhiza statistically reduced irrigation water use by 50% without affecting plant DM production.