Real-time PCR assay to detect the novel Clade Ib monkeypox virus, September 2023 to May 2024
Monkeypox virus (MPXV) is an emerging zoonotic pathogen with complex epidemiology necessitating rapid diagnosis and distinguishing between clades and subclades. The emerging Clade Ib lacks the genomic region used in the Clade I-specific assay from the Centers for Disease Control and Prevention. We report an MPXV real-time PCR to specifically detect Clade Ib. The assay demonstrated proficient sensitivity and specificity in 92 samples and can be included along other TaqMan-based assays to detect MPXV and distinguish between clades and subclades.
Authors
- Marion Koopmans (ORCID: https://orcid.org/0000-0002-5204-2312)
- Pacifique Ndishimye (ORCID: https://orcid.org/0000-0003-3285-2622)
- Frank M. Aarestrup (ORCID: https://orcid.org/0000-0002-7116-2723)
- Richard Molenkamp (ORCID: https://orcid.org/0000-0002-9004-3850)
- Leonard Schuele (ORCID: https://orcid.org/0000-0003-1721-5879)
- Bas B. Oude Munnink (ORCID: https://orcid.org/0000-0002-9394-1189)
- Jean Claude Udahemuka (ORCID: https://orcid.org/0000-0001-8370-7113)
- Justin Bengehya Mbiribindi
- Freddy Belesi Siangoli
- Leandre Murhula Masirika (ORCID: https://orcid.org/0009-0000-0688-5688)
Institutions
- Erasmus MC (NL)
- University of Rwanda (RW)
- Instituto de Investigación en Recursos Cinegéticos (ES)
- African Institute for Mathematical Sciences (RW)
- Collaborative Neuroscience Network (US)
- University of Castilla-La Mancha (ES)
- Technical University of Denmark (DK)
Publication Details
- Journal
- Eurosurveillance
- Published
- 2024-08-08
- DOI
- https://doi.org/10.2807/1560-7917.es.2024.29.32.2400486
- Citations
- 61
- Primary Topic
- Poxvirus research and outbreaks
- Type
- article
- Field-Weighted Citation Impact
- 17.91
Funders
- Conservation Action Research Network
- University of Oxford