Understanding natural isotopic variations in cultured cancer cells
Exposing cultured cancer cells to hypoxia allowed us to further investigate the relation between metabolic modulations and natural isotopic variations, while mitigating the confounding impact of changing culture medium composition. This study highlights the potential of natural δ<sup>13</sup>C variations for studying substrate fluxes and nutrient allocation in reproducible culture conditions. Considering cell yield and culture medium composition is pivotal to the success of this approach.
Authors
- R. Hankard (ORCID: https://orcid.org/0000-0001-8450-5839)
- A. De Luca (ORCID: https://orcid.org/0000-0003-1704-8599)
- Karine Mahéo
- Gaëlle Fromont (ORCID: https://orcid.org/0000-0002-7730-2264)
- Olivier L. Mantha (ORCID: https://orcid.org/0000-0002-4446-9674)
- Maxime Guéguinou (ORCID: https://orcid.org/0000-0002-5793-2082)
- Marie Mahé
- Illa Tea
Institutions
- Université de Tours (FR)
- Centre National de la Recherche Scientifique (FR)
- Inserm (FR)
- Institut de Cancérologie de l'Ouest (FR)
- Chimie et Interdisciplinarité, Synthèse, Analyse, Modélisation (FR)
- Nantes Université (FR)
Publication Details
- Journal
- Rapid Communications in Mass Spectrometry
- Published
- 2024-08-08
- DOI
- https://doi.org/10.1002/rcm.9878
- Citations
- 3
- Primary Topic
- Cancer, Hypoxia, and Metabolism
- Type
- article
- Field-Weighted Citation Impact
- 0.86