Protocol for fluorescent live-cell staining of tardigrades
Tardigrades are microscopic organisms with exceptional resilience to environmental extremes. Most protocols to visualize the internal anatomy of tardigrades rely on fixation, hampering our understanding of dynamic changes to organelles and other subcellular components. Here, we provide protocols for staining live tardigrade adults and other postembryonic stages, facilitating real-time visualization of structures including lipid droplets, mitochondria, lysosomes, and DNA.
Authors
- Jonathan D. Hibshman (ORCID: https://orcid.org/0000-0001-7324-9382)
- Clayton J. Harry (ORCID: https://orcid.org/0000-0003-2441-9191)
- Jake Leyhr (ORCID: https://orcid.org/0000-0003-1815-7818)
- Marycruz Flores-Flores
- Martín A. Estermann (ORCID: https://orcid.org/0000-0002-8623-2720)
- Jorge Lázaro (ORCID: https://orcid.org/0000-0002-9901-356X)
- Elizabeth-Ann Legere (ORCID: https://orcid.org/0000-0002-8603-1662)
- Siddharthan Balachandar Thendral (ORCID: https://orcid.org/0000-0002-4674-7033)
- Bob Goldstein (ORCID: https://orcid.org/0000-0001-6961-675X)
- Amor Damatac
- Bridget A Vincent
- Phillip L Davidson
- Caroline M Holmes
Institutions
- University of North Carolina at Chapel Hill (US)
- Marine Biological Laboratory (US)
- University of California, Santa Barbara (US)
- National Institute of Environmental Health Sciences (US)
- European Molecular Biology Laboratory (ES)
- Indiana University Bloomington (US)
- Max Planck Institute for Evolutionary Biology (DE)
- University of Colorado Anschutz Medical Campus (US)
Publication Details
- Journal
- STAR Protocols
- Published
- 2024-08-09
- DOI
- https://doi.org/10.1016/j.xpro.2024.103232
- Citations
- 3
- Primary Topic
- Tardigrade Biology and Ecology
- Type
- article
- Field-Weighted Citation Impact
- 3.09
Funders
- National Science Foundation