Alternating between even and odd ploidy levels switches on and off the recombination control, even near the centromeres
Meiotic recombination is a key biological process in plant evolution and breeding, as it generates genetic diversity in each generation through the formation of crossovers (COs). However, due to their importance in genome stability, COs are highly regulated in frequency and distribution. We previously demonstrated that this strict regulation of COs can be modified, both in terms of CO frequency and distribution, in allotriploid Brassica hybrids (2n = 3x = 29; AAC) resulting from a cross between Brassica napus (2n = 4x = 38; AACC) and Brassica rapa (2n = 2x = 20; AA). Using the recently updated B. napus genome now including pericentromeres, we demonstrated that COs occur in these cold regions in allotriploids, as close as 375 kb from the centromere. Reverse transcription quantitative PCR (RT-qPCR) of various meiotic genes indicated that Class I COs are likely involved in the increased recombination frequency observed in allotriploids. We also demonstrated that this modified recombination landscape can be maintained via successive generations of allotriploidy (odd ploidy level). This deregulated meiotic behavior reverts to strict regulation in allotetraploid (even ploidy level) progeny in the second generation. Overall, we provide an easy way to manipulate tight recombination control in a polyploid crop.
Authors
- Olivier Martin (ORCID: https://orcid.org/0000-0002-5295-5963)
- Marie Gilet
- Virginie Huteau (ORCID: https://orcid.org/0009-0003-8034-4320)
- Karine Labadie (ORCID: https://orcid.org/0000-0001-7467-8509)
- Caroline Belser (ORCID: https://orcid.org/0000-0002-8108-9910)
- Gwenaëlle Deniot (ORCID: https://orcid.org/0009-0004-1196-3256)
- Anael Brunet (ORCID: https://orcid.org/0009-0009-9929-7185)
- Jérôme Morice (ORCID: https://orcid.org/0009-0006-8033-2022)
- Gwenn Trotoux (ORCID: https://orcid.org/0009-0003-1261-0463)
- Anne‐Marie Chèvre (ORCID: https://orcid.org/0000-0001-5312-032X)
- Cyril Falentin (ORCID: https://orcid.org/0000-0002-9901-3412)
- Mathieu Rousseau‐Gueutin (ORCID: https://orcid.org/0000-0002-1130-1090)
- Jean‐Marc Aury (ORCID: https://orcid.org/0000-0003-1718-3010)
- Franz Boideau (ORCID: https://orcid.org/0000-0001-5967-8746)
- Matthieu Falque (ORCID: https://orcid.org/0000-0002-6444-858X)
- Julien Boutte (ORCID: https://orcid.org/0009-0005-2567-6386)
- Gautier Richard (ORCID: https://orcid.org/0000-0003-3663-4569)
- Olivier Coriton (ORCID: https://orcid.org/0000-0003-3741-447X)
- Cécile Baron (ORCID: https://orcid.org/0000-0002-3713-370X)
- Loeiz Maillet (ORCID: https://orcid.org/0000-0002-8686-5982)
- Maryse Lodé-Taburel (ORCID: https://orcid.org/0009-0003-9756-0740)
- Frédérique Eber
Institutions
- Centre National de la Recherche Scientifique (FR)
- Université Paris Cité (FR)
- AgroParisTech (FR)
- Commissariat à l'Énergie Atomique et aux Énergies Alternatives (FR)
- Université Paris-Saclay (FR)
- Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR)
- Génétique Quantitative et Évolution Le Moulon (FR)
- Genoscope (FR)
- CEA Paris-Saclay (FR)
- Institut de Génétique, Environnement et Protection des Plantes (FR)
- Université de Rennes (FR)
Publication Details
- Journal
- The Plant Cell
- Published
- 2024-08-09
- DOI
- https://doi.org/10.1093/plcell/koae208
- Citations
- 4
- Primary Topic
- Chromosomal and Genetic Variations
- Type
- article
- Field-Weighted Citation Impact
- 2.40
Funders
- Agence Nationale de la Recherche
- Région Bretagne
- Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement