Metabolic features of neutrophilic differentiation of HL-60 cells in hyperglycemic environments
HL-60 neutrophil-like differentiation recapitulated known molecular and metabolic features of human neutrophil differentiation. Increased glucose concentrations correlated with features described in hyperglycemic donor neutrophils including increased CD11b and phagocytosis. We used this model to describe metabolic features of neutrophil-like cell differentiation in hyperglycemia and show for the first time the downregulation of CPT1 and citrate synthase activity, independently of mitochondrial mass.
Authors
- Heriberto Prado-Garcı́a (ORCID: https://orcid.org/0000-0002-4932-3244)
- José Antonio Cruz-Cárdenas
- Luis Alberto Luévano‐Martínez (ORCID: https://orcid.org/0000-0003-2598-7967)
- Marion E. G. Brunck (ORCID: https://orcid.org/0000-0001-8994-8276)
- Alejandra López-Arredondo (ORCID: https://orcid.org/0000-0003-3557-5122)
- Gerardo García‐Rivas (ORCID: https://orcid.org/0000-0003-4731-3293)
- Jorge Andrés Cázares-Preciado (ORCID: https://orcid.org/0000-0003-2661-584X)
Institutions
- Instituto Nacional de Enfermedades Respiratorias (MX)
- Tecnológico de Monterrey (MX)
Publication Details
- Journal
- BMJ Open Diabetes Research & Care
- Published
- 2024-08-01
- DOI
- https://doi.org/10.1136/bmjdrc-2024-004181
- Citations
- 4
- Primary Topic
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Type
- article
- Field-Weighted Citation Impact
- 0.96
Funders
- Instituto Tecnológico y de Estudios Superiores de Monterrey