Development of a media cell-free DNA direct digital PCR method for cell viability estimation
The developed media cfDNA direct dPCR method allows for efficient measurements of the degree of cell viability. Unlike other conventional cell-based assays, our method has advantages of no loss of cultured cells and the ability to implement online analysis. Accurate and sensitive media cfDNA analysis using dPCR can be adopted in various applications such as determining cytotoxicity levels in large-scale bioreactors or screening for effective anticancer drugs.
Authors
- Young‐Kyung Bae (ORCID: https://orcid.org/0000-0001-5293-8752)
- Ah Leum Kim
- Hye Ji Shin
- Ji Youn Lee (ORCID: https://orcid.org/0000-0002-4288-4559)
Institutions
- Chungnam National University (KR)
- Korea Research Institute of Standards and Science (KR)
- Sungkyunkwan University (KR)
Publication Details
- Journal
- Analytica Chimica Acta
- Published
- 2024-08-08
- DOI
- https://doi.org/10.1016/j.aca.2024.343064
- Citations
- 2
- Primary Topic
- Molecular Biology Techniques and Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.47
Funders
- Ministry of Food and Drug Safety
- Ministry of Science, ICT and Future Planning
- Korea Research Institute of Standards and Science
- Ministry of Science and ICT, South Korea