Investigation and Mathematical Modelling of Anaerobic Biofermentation of Dairy Cattle Manure: Influence of Addition of Citrus Peel Waste and Lycoperdon Perlatum Spores

Abstract The production of biogas from organic and carbon-neutral sources through biomethanization is essential for achieving the goals of the circular economy and reducing carbon emissions. Crucial for the optimal efficiency of anaerobic biofermentation processes is the influence of organic substrate and the application of methanogenesis enhancement additives. This study explores the possibilities of using a substrate of citrus biomass (waste peelings) for methane production from dairy cattle manure, as well as the influence of an addition of puffball mushroom (Lycoperdon perlatum) spores on the process. Experimental data obtained over a period of 31 days were mathematically modelled using a Verhulst logistic growth model to forecast their future development. The results indicate an inhibitory effect of citrus biomass on methanogenesis processes with a 20% lower yield, but a positive effect of Lycoperdon perlatum spores with nearly double the amount of biogas produced and higher methane content.