Preparation and optimization of niosome encapsulated meropenem for significant antibacterial and anti-biofilm activity against methicillin-resistant Staphylococcus aureus isolates
An F1 formulation of niosome-encapsulated meropenem with a size of 51.3 ± 5.84 nm and an encapsulation efficiency of 84.86 ± 3.14 % was achieved. The synthesized niosomes prevented biofilm capacity with a biofilm growth inhibition index of 69 % and significantly downregulated <i>icaD, FnbA, Ebps</i>, and <i>Bap</i> gene expression in MRSA strains (p < 0.05). In addition, the F1 formulation increased antibacterial activity by 4-6 times compared with free meropenem. Interestingly, the F1 formulation of niosome-encapsulated meropenem indicated cell viability >90 % at all tested concentrations against normal HDF cells. The results of the present study indicate that niosome-encapsulated meropenem increased antibacterial and anti-biofilm activities without profound cytotoxicity in normal human cells, which could prove useful as a good drug delivery system.
Authors
- Amir Mirzaie (ORCID: https://orcid.org/0000-0002-0941-7828)
- Erfan Ghanbarzadeh (ORCID: https://orcid.org/0000-0002-1046-834X)
- Kamal Paseban
- Farnoush Falahi Robattorki
- Romina Andalibi
- Arezou Azari
- Abbas Haddadian
- Aliasghar Bagheri Kashtali
- Hedieh Dibah
- Reza Amani (ORCID: https://orcid.org/0000-0002-0074-4080)
- Arman Chitgarzadeh
- Tohid Piri Gharaghie (ORCID: https://orcid.org/0000-0002-0041-5842)
- Shadi Hajrasouiha
- Tina Rashidian
- Zahra Pirdolat
- Massoumeh Salarkia
- Dorsa Sadat Shahrava
- Fatemeh Safaeinikjoo
- Sama Noroozi
- Niloofar Saeinia
- Ali Ahmadiyan
- Roza Mazid Abadi
- Rokhshad Gharehcheloo
- Fatima Sabouri
- Faezeh Sadat Kermani
- Niusha Sadat Hosseini
- Atena Seifi
- Aryan Aryan Tavana
Institutions
- Islamic Azad University Roudehen Branch (IR)
- Islamic Azad University of Zanjan (IR)
- Islamic Azad University South Tehran Branch (IR)
- Tarbiat Modares University (IR)
- Islamic Azad University, Science and Research Branch (IR)
- Guilan University of Medical Sciences (IR)
- University of Utah (US)
- Islamic Azad University of Parand (IR)
- Biotechnology Research Center (IR)
- Islamic Azad University of Shahrekord (IR)
- Islamic Azad University Pharmaceutical Sciences Branch (IR)
Publication Details
- Journal
- Heliyon
- Published
- 2024-08-01
- DOI
- https://doi.org/10.1016/j.heliyon.2024.e35651
- Citations
- 20
- Primary Topic
- Advancements in Transdermal Drug Delivery
- Type
- article
- Field-Weighted Citation Impact
- 4.16
Funders
- Islamic Azad University, Ahar Branch
- Islamic Azad University of Shahrekord
- Islamic Azad University
- Islamic Azad University, Shahrekord