Kinetochore and ionomic adaptation to whole-genome duplication in Cochlearia shows evolutionary convergence in three autopolyploids
Whole-genome duplication (WGD) occurs in all kingdoms and impacts speciation, domestication, and cancer outcome. However, doubled DNA management can be challenging for nascent polyploids. The study of within-species polyploidy (autopolyploidy) permits focus on this DNA management aspect, decoupling it from the confounding effects of hybridization (in allopolyploid hybrids). How is autopolyploidy tolerated, and how do young polyploids stabilize? Here, we introduce a powerful model to address this: the genus Cochlearia, which has experienced many polyploidization events. We assess meiosis and other polyploid-relevant phenotypes, generate a chromosome-scale genome, and sequence 113 individuals from 33 ploidy-contrasting populations. We detect an obvious autopolyploidy-associated selection signal at kinetochore components and ion transporters. Modeling the selected alleles, we detail evidence of the kinetochore complex mediating adaptation to polyploidy. We compare candidates in independent autopolyploids across three genera separated by 40 million years, highlighting a common function at the process and gene levels, indicating evolutionary flexibility in response to polyploidy.
Authors
- Patrick J. Monnahan (ORCID: https://orcid.org/0000-0002-3022-8191)
- Stuart D. Desjardins (ORCID: https://orcid.org/0000-0002-3749-1873)
- Sina Fischer (ORCID: https://orcid.org/0000-0001-8368-3262)
- James D. Higgins (ORCID: https://orcid.org/0000-0001-6027-8678)
- Eva Wolf (ORCID: https://orcid.org/0000-0001-6319-2538)
- Мarcus A. Koch (ORCID: https://orcid.org/0000-0002-1693-6829)
- Levi Yant (ORCID: https://orcid.org/0000-0003-3442-0217)
- Filip Kolář (ORCID: https://orcid.org/0000-0002-8793-7992)
- Martin A. Lysák (ORCID: https://orcid.org/0000-0003-0318-4194)
- Terezie Mandáková (ORCID: https://orcid.org/0000-0001-6485-0563)
- Tuomas Hämälä (ORCID: https://orcid.org/0000-0001-8306-3397)
- Min Zhou (ORCID: https://orcid.org/0000-0002-9711-8894)
- Jordan Koch (ORCID: https://orcid.org/0000-0002-9785-5878)
- Christopher Moore (ORCID: https://orcid.org/0000-0002-3122-7502)
- Thomas C. Mathers (ORCID: https://orcid.org/0000-0002-8637-3515)
- Sian Bray (ORCID: https://orcid.org/0000-0001-5411-8248)
- Laura Cowan
- Sílvia Busoms (ORCID: https://orcid.org/0000-0002-9917-7118)
Institutions
- John Innes Centre (GB)
- Central European Institute of Technology (CZ)
- University of Nottingham (GB)
- University of Leicester (GB)
- Czech Academy of Sciences (CZ)
- Charles University (CZ)
- Heidelberg University (DE)
- Norwich Research Park (GB)
- Central European Institute of Technology – Masaryk University (CZ)
Publication Details
- Journal
- Cell Reports
- Published
- 2024-08-01
- DOI
- https://doi.org/10.1016/j.celrep.2024.114576
- Citations
- 15
- Primary Topic
- Genomics and Phylogenetic Studies
- Type
- article
- Field-Weighted Citation Impact
- 3.27
Funders
- Leverhulme Trust
- European Commission
- Ministerstvo Školství, Mládeže a Tělovýchovy
- Directorate for Biological Sciences
- Horizon 2020 Framework Programme
- Biotechnology and Biological Sciences Research Council
- European Research Council
- Horizon 2020
- H2020 European Research Council
- H2020 Marie Skłodowska-Curie Actions
- HORIZON EUROPE Marie Sklodowska-Curie Actions