A rapid LAMP assay for the diagnosis of oak wilt with the naked eye
In summary, the LAMP assay coupled with oligonucleotide-conjugated gold nanoparticle visualization is a promising method for accurate and rapid molecular-based diagnosis of B. fagacearum in field settings. The new method can be adapted to other forest and plant diseases by simply designing new primers.
Authors
- Jennifer Juzwik (ORCID: https://orcid.org/0000-0003-0577-7807)
- Hamada A. Aboubakr (ORCID: https://orcid.org/0000-0002-8233-2353)
- Vinni Thekkudan Novi (ORCID: https://orcid.org/0000-0001-7236-5832)
- Melanie J. Moore (ORCID: https://orcid.org/0000-0002-5894-3752)
- Akli Zarouri
- Abdennour Abbas (ORCID: https://orcid.org/0000-0003-1957-8948)
Institutions
- University of Minnesota (US)
- Biotechnology Institute (US)
- Northern Research Station (US)
Publication Details
- Journal
- Plant Methods
- Published
- 2024-08-05
- DOI
- https://doi.org/10.1186/s13007-024-01254-8
- Citations
- 7
- Primary Topic
- Biosensors and Analytical Detection
- Type
- article
- Field-Weighted Citation Impact
- 1.29
Funders
- National Science Foundation
- U.S. Department of Agriculture
- University of Minnesota
- National Institute of Food and Agriculture
- Materials Research Science and Engineering Center, Harvard University
- Minnesota Invasive Terrestrial Plants and Pests Center, University of Minnesota