Human induced pluripotent stem cells for live cell cycle monitoring and endogenous gene activation

The fluorescence ubiquitination cell cycle inhibitor (FUCCI) has been introduced to monitor cell cycle activity in living cells, including human induced pluripotent stem cells (hiPSC) and derived cell types. We have recently developed hiPSC with stable expression of dCas9VPR for endogenous gene activation and a Citrine-tagged ACTN2 cell line to monitor sarcomere development and function in muscle cells. Here, we present dual and triple transgenic hiPSC lines developed by genomic integration of FUCCI with and without dCas9VPR into the ROSA26 and AAVS1 loci, respectively, in the previously introduced ACTN2-Citrine line. Functionality of the transgenes was demonstrated in the novel hiPSC line, which we introduce as Myo-CCER and CraCCER.

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Publication Details

Journal
Stem Cell Research
Published
2024-08-05
DOI
https://doi.org/10.1016/j.scr.2024.103531
Citations
1
Primary Topic
Pluripotent Stem Cells Research
Type
article
Field-Weighted Citation Impact
0.23

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Human induced pluripotent stem cells for live cell cycle monitoring and endogenous gene activation

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Human induced pluripotent stem cells for live cell cycle monitoring and endogenous gene activation

Eric Schoger, Norman Y. Liaw, Laura C. Zelarayán, Rosa Kim, Wolfram‐H. Zimmermann, Sebastian H. Nagel
article en
1 citations

Abstract

The fluorescence ubiquitination cell cycle inhibitor (FUCCI) has been introduced to monitor cell cycle activity in living cells, including human induced pluripotent stem cells (hiPSC) and derived cell types. We have recently developed hiPSC with stable expression of dCas9VPR for endogenous gene activation and a Citrine-tagged ACTN2 cell line to monitor sarcomere development and function in muscle cells. Here, we present dual and triple transgenic hiPSC lines developed by genomic integration of FUCCI with and without dCas9VPR into the ROSA26 and AAVS1 loci, respectively, in the previously introduced ACTN2-Citrine line. Functionality of the transgenes was demonstrated in the novel hiPSC line, which we introduce as Myo-CCER and CraCCER.

Stem Cell ResearchVol. 80
Justus-Liebig-Universität Gießen (DE), Heidelberg University (DE), German Center for Neurodegenerative Diseases (DE), University Hospital Heidelberg (DE), Fraunhofer Institute for Translational Medicine and Pharmacology (DE), Universitätsmedizin Göttingen (DE), German Centre for Cardiovascular Research (DE), Nanoscale Microscopy and Molecular Physiology of the Brain Cluster of Excellence 171 — DFG Research Center 103 (DE), Multiscale Bioimaging, University of Göttingen (DE)
Deutsches Zentrum für Herz-Kreislaufforschung, Universitätsmedizin Göttingen, Deutsche Forschungsgemeinschaft, Bundesministerium für Bildung und Forschung, Deutsche Gesellschaft für Kardiologie-Herz und Kreislaufforschung., National Institutes of Health, National Center for Advancing Translational Sciences, Common Fund
Zero hunger
Openalex Percentile: Top 43%
Pluripotent Stem Cells Research
0.23
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