Peripheral and seminal biochemical profiles in relation to sperm kinematics across seasons in buffalo bulls

The correlation between peripheral and seminal hormones and biochemical parameters with sperm kinematics across seasons in buffalo bulls were studied. Semen from buffalo bulls (n = 13) during different seasons viz. summer, comfort (spring and autumn) and winter was collected. Sperm kinematics of ejaculates (n = 39) (three ejaculates per bull) were carried out during each season and simultaneously blood (10 mL) was collected and serum was separated for hormones (leptin, testosterone and kisspeptin) and biochemical [heat shock protein 70 (HSP70), ascorbic acid and total antioxidant capacity] estimation. Total motility (TM, %), progressive motility (PM, %) and rapid motility (RM, %) showed significant differences across seasons with the highest TM, PM and RM during winter. HSP70 was higher in seminal plasma levels during summer and TAC was higher in serum than seminal plasma during all seasons. In conclusion, seminal leptin and ascorbic acid showed an association with sperm kinematics across seasons in buffalo bulls.