Toxicity Equivalency Factors for Tetrodotoxin Analogues Determined with Automated Patch Clamp on Voltage-Gated Sodium Channels in Neuro-2a Cells
Tetrodotoxin (TTX) is a potent marine neurotoxin, responsible for numerous poisoning incidents and some human fatalities. To date, more than 30 TTX analogues have been identified, but their individual toxicities and roles in poisoning remain largely unknown. In this work, the toxicity equivalency factors (TEFs) of five TTX analogues were determined by assessing the blockade of voltage-gated sodium channels in Neuro-2a cells using automated patch clamp (APC). All TTX analogues were less toxic than TTX. The derived TEFs were applied to the individual TTX analogues concentrations measured in pufferfish samples, using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). A comparison of these results with those obtained from APC analysis demonstrated that TEFs can be effectively used to translate LC-MS/MS analytical data into meaningful toxicological information. This is the first study to utilize APC device for the toxicological assessment of TTX analogues, highlighting its potential as a bioanalytical tool for seafood safety management and human health protection.
Authors
- Francesc X. Sureda (ORCID: https://orcid.org/0000-0002-7968-3929)
- Jordi Molgó (ORCID: https://orcid.org/0000-0002-0693-8994)
- Jorge Diogène (ORCID: https://orcid.org/0000-0002-6567-6891)
- Andres Sanchez‐Henao (ORCID: https://orcid.org/0000-0003-3413-4755)
- Jaume Reverté (ORCID: https://orcid.org/0009-0002-6914-2851)
- Manolis Mandalakis (ORCID: https://orcid.org/0000-0002-7469-4290)
- Maria Rambla-Alegre (ORCID: https://orcid.org/0000-0003-4276-276X)
- Panagiota Peristeraki (ORCID: https://orcid.org/0000-0002-8608-078X)
- Mònica Campàs (ORCID: https://orcid.org/0000-0002-1220-7100)
Institutions
- Universidad de Las Palmas de Gran Canaria (ES)
- Centre National de la Recherche Scientifique (FR)
- Commissariat à l'Énergie Atomique et aux Énergies Alternatives (FR)
- Université Paris-Saclay (FR)
- Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR)
- Institute of Agrifood Research and Technology (ES)
- CEA Paris-Saclay (FR)
- Hellenic Centre for Marine Research (GR)
- Universitat Rovira i Virgili (ES)
Publication Details
- Journal
- Journal of Agricultural and Food Chemistry
- Published
- 2024-08-05
- DOI
- https://doi.org/10.1021/acs.jafc.4c04321
- Citations
- 5
- Primary Topic
- Marine Toxins and Detection Methods
- Type
- article
- Field-Weighted Citation Impact
- 1.25
Funders
- Ministerio de Ciencia e Innovación
- Agència de Gestió d'Ajuts Universitaris i de Recerca