Monitoring sulfate-reducing bacteria (SRB) using molecular tools during in situ immobilization of heavy metals

In situ bioprecipitation (ISBP) of heavy metals as metal sulfides by the activity of sulfatereducing bacteria (SRB) is a promising strategy for sustaining groundwater quality. The feasibility of the ISBP process for a Zn,Cd,Ni and Co -polluted industrial site was studied in column experiments, using lactate, molasses or HRCⓇ as supplemented electron donor and C -source. In addition, the effect of extra N/P was evaluated. In order to link the results of analytical analyzes to SRB-community composition and activity, the SRB population was monitored by molecular tools such as SRB-subgroup or – genus specific PCR and DGGE of the 16S rRNA- and dsr(dissimilatory sulfite reductase) gene.