The Use of Microparticulate Reversed-Phase Packing in High-Pressure Liquid Chromatography of Compounds of Biological Interest

Chemically bonded stationary phases for use in high-pressure liquid chromatography (HPLC) were first introduced in 1970. These chemically bonded phases are liquids or solids chemically attached to the support material, visually silica gel, and oriented like bristles on the column of support [1, 2]. Column efficiencies, as measured by plates per meter, are much higher on the chemically bonded phases than on the conventionally coated phases in which liquids or solids are deposited on the support and held by physical forces. In addition, these permanently bonded phases obviate the need for using pre satura ted solvents and eliminate column bleed. In 1974, the column efficiency of these packings was further increased by the use of supports of very small particle size: 5 to 10 μm.